SELDI-TOF -System 4000 Enterprise
The main use of this technology is in rapid protein biomarker discovery from body fluids, cell/tissue extracts or cell cultures. Alternatively it is used for the mass analysis of compounds such as proteins, peptides and nucleic acids especially when they are present in complex biological media.
SELDI operates by capturing compound(s) of interest on a chip, the surface of which possesses, or is made to possess through chemical coupling, affinity characteristics such as ion-exchange, hydrophobicity, antigen/antibody etc. Compounds of interest dock onto the surface through the selected affinity interactions whilst non-binding "contaminants" are removed by washing. The captured substances (which are non-covalently bound) are then analysed by MALDI-ToF Mass Spectrometry which provides molecular weight information. The technique can be used to identify protein–protein, antibody-antigen etc. interactions. Once molecular-docking is achieved, the mass spectra are usually obtained within minutes.
The ProteinChip SELDI system uses SELDI technology to quickly deliver protein mass profiles from complex biological samples. The fully integrated platform consists of ProteinChip arrays, instrumentation (the ProteinChip SELDI reader), and software. ProteinChip arrays bind whole classes of proteins present in complex samples, and these proteins are then detected by the ProteinChip SELDI reader. The software facilitates data collection and analysis.
The ProteinChip SELDI reader enables analysis of minute amounts of protein selectively captured on a ProteinChip array. Designed with proprietary technology, the reader takes advantage of time-of-flight (TOF) mass spectrometry to determine the precise molecular weights of multiple proteins from native biological samples.